I was born & brought up in Sivakasi, Tamil Nadu and have done my studies as well. I have completed both UG and PG Botany from ANJA College, Madurai Kamaraj University and I have done my PhD in Botany from Bharathidasan University, Thiruchirrappli, Tamil Nadu. For my PhD work, I have developed fungal disease tolerant genetically modified cotton plants by transforming rice chitinase gene. After my graduation, I worked in Jeju National University, South Korea for 3 years and 6 months as post doctoral research scientist in the field of genetic engineering. I developed different turfgrass species by transforming phytochrome and bar genes. Later, I worked as a JSPS-Post Doctoral Fellow in Gifu University, Gifu, Japan in the field of Cotton Molecular Biology. During my career, I visited different countries related to academic and research programs. My experience to date covers various plant biology and plant molecular biological techniques, which will be useful to teach and train young students.
B.Sc. (Botany), 1996 – 1999, MK University, Madurai, Tamil Nadu, India
M.Sc. (Botany), 1999 – 2001, MK University, Madurai, Tamil Nadu, India
Ph.D. (Botany), 2002- 2008, Department of Plant Science, Bharathidasan University, Trichy, Tamil Nadu, India. Title : In vitro culture, selection and Agrobacterium tumefaciens - mediated chitinase gene (chi II) transfer studies in cotton (Gossypium hirsutum L) for improved fungal disease tolerance.
PDF (Genetic Engineering), 2008 - 2011, Faculty of Biotechnology, Jeju National University, Jeju, South Korea. Title : Development of transgenic turfgrasses by using Phytochrome genes.
JSPS-PDF (Genetic Engineering), 2011 - 2013, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan. Title : Characterization of cotton STOP1 system against acid soil conditions for molecular breeding.
I have completed both UG and PG Botany from Madurai Kamaraj University and I have done my PhD in Botany from Bharathidasan University, Thiruchirrappli, Tamil Nadu. I have developed fungal disease tolerant genetically modified cotton plants by transforming rice chitinase gene. After my graduation, I worked in Jeju National University, South Korea for 3 years and 6 months as post doctoral research scientist in the field of genetic engineering of turfgrass varieties by using phytochrome and bar genes. Later, I worked as a JSPS-Post Doctoral Fellow in Gifu University, Gifu, Japan in the field of Cotton Molecular Biology. During my career, I visited different countries related to academic and research programs. My experience to date covers various plant biology and plant molecular biological techniques, which will be useful to teach and train young students.
Research / Administrative Experience+
Development of transgenic plants for enhanced resistance against different abiotic and biotic stress factors.
In order to develop acid soil tolerant cotton, we targeted to understand the role of GhSTOP1 (sensitivity to proton rhizotoxicity1) system involved in acid soil rhizotoxicity tolerance of cotton plants. Based on the conserved zinc finger domain of AtSTOP1, we cloned cotton STOP1 (GhSTOP1). Transcriptome analysis displayed that various genes were down regulated in the Arabidopsis STOP1 mutant and suggested that STOP1 is involved in regulation of signal transduction pathways of Al and H+ responsive gene expression. Based on these observations, we transformed GhSTOP1 gene under control of CaMV35S promoter into cotton for over-expression. Furthermore, we also transformed GhSTOP1-RNAi cassette into cotton to down-regulate the GhSTOP1. An update on genotypic and phenotypic variations and recent progress made in the transgenic cells expressing GhSTOP1 or GhSTOP1-RNAi cassettes will be analyzed by using advanced methods. Meantime, we also targeted to clone the GhALS3 and GhMATE gene(s) in order to understand their roles towards acid soil tolerance.
I am also working with Characterization of transgenic cotton plants over-expressing hyperactive mutant Phytochrome A (Phy A). Phytochrome A (phyA) in higher plants functions as a far-red/shade light-sensing photoreceptor to suppress the shade-avoidance responses to shade stress. Since the overexpression of phytochromes in crop plants used to overcome crop yield losses from shade avoidance reactions, the PHYA gene has been introduced into crop plants such as tobacco, tomato, potato, rice, and wheat. In this study, we targeted to introduce the Avena sativa (oat) PHYA gene along with the bar gene into cotton. Furthermore, a hyperactive mutant of the Avena sativa PHYA gene in which a phosphorylation site involved in light-signal attenuation was removed by changing a serine to an alanine residue (Ser599Ala) will also be transformed into cotton. Apparent phenotypes will be compared to evaluate the shade-avoidance responses under different light conditions.
Teaching / Other Experience+
1. Plant Molecular Biology: Gene cloning, Vector contruction for Overexpression and RNAi mediated downregulation, Plant genetic transformation methods,
2. Plant Biotechnology: Plant Cell and Tissue and Organ Culture, Somatic Embryogenesis and Organogenesis.
Post Graduate Supervision+
My research work is focused on the development and characterization of transgenic plants (Cotton and Legumes) towards abiotic stress tolerance.
Currently the following two students are registered for PhD.
1. Ms.ATREYEE KUNDU (Qualified with GATE - Percentile 92)
Title : Development of transgenic Cotton Plants for improved acid soil tolerance.
Duration : May’ 2015 to June 2018.
2. Mr. SUBHARTHI DAS
Title : Acid soil tolerance studies in Mungbean.
Duration : May 2015 to June 2018.
3. Karishma Agarwal,
Title : acid soil tolerance studies in Azolla plants.
Duration : April 2018 to March 2021.
1. International Society for Root Research
2. American Society for Plant Biologists
3. Japanese Society for Plant Nutrition and Soil Science.
4. Swamy Botanical Club, India (Life Member).
5. Indian Science Congress Association, India (Life Member)
1. Ganesan M, Lee HY, Kim JI, Song PS (2017) Development of transgenic crops based on photo-biotechnology, Plant, Cell and Environment, 40, 2469–2486. IF : 6.17
2. Gururani MA, Ganesan M, Song IJ, Han Y, Kim JI, Lee HY, Song PS, (2016) Transgenic Turfgrasses Expressing Hyperactive Ser599Ala Phytochrome A Mutant Exhibit Abiotic Stress Tolerance. Journal of Plant Growth Regulation, 35:11-21, IF : 2.16
3. Gururani MA, Ganesan M, and Song PS (2015) Photo-biotechnology as a tool to improve agronomic traits in crops. Biotechnology Advances, 33: 53-63, IF:10.59
4. Gururani MA, Venkatesh J, Ganesan M, Strasser RJ, Song IJ, Han Y, Kim JI, Lee HY, Song PS, In vivo assessment of cold tolerance through chlorophyll-a fluorescence in transgenic zoysiagrass plants expressing mutant phytochrome A (2015) Plos One, 10(5): e0127200, IF : 3.03
5. Kobayashi Y, Ohyama Y, Kobayashi Y, Ito H, Iuchi S, Fujita M, Zhao CR, Tanveer T, Ganesan M, Kobayashi M, and Koyama H (2014) STOP2 activates transcription of several genes for Al- and low pH-tolerance that are regulated by STOP1 in Arabidopsis, Molecular Plant, 7(2): 311-322. IF:8.8
6. In-Ja Song, Tae-Woong Bae, Ganesan M, Jeong-Il Kim, Hyo-Yeon Lee and Pill-Soon Song (2013) Transgenic Herbicide-Resistant Turfgrasses In: Herbicides; Editors A Price and J Kelton, Intech Publications, USA, pp 377 – 396.
7. Ganesan M, Han YJ, Bae TW, Hwang OJ, Chandrasekkhar T, Shin AY, Goh CH, Nishiguchi S, Song IJ, Lee HY, Kim JI, and Pill-Soon Song (2012) Overexpression of phytochrome A and its hyperactive mutant improves shade tolerance and turf quality in creeping bentgrass and zoysiagrass. Planta, 236:1135–1151. IF:3.36
8. Zhang JY, Bae TW, Boo KH, Sun HJ, Song IJ, Pham CH, Ganesan M, Yang DH, Kang HG, Ko SM, Riu KZ, Lim PO, and HY Lee (2011) Ginsenoside production and morphological characterization of wild ginseng (Panax ginseng Meyar) mutant lines induced by g-irradiation (60Co) of adventitious roots. J. Ginseng Research, 35 (3): 283- 293. IF: 4.08
9. Song IJ, Ganesan M, Kang EJ, Sun HJ, Bae TW, Lim PO, Song PS, and Lee HY (2010) A simple and reproducible regeneration protocol for Zoysia japonica based on callus cultures. Horticulture, Environment and Biotechnology, 51(3):222-225. IF:0.8
10. Ganesan M, Bhanumathi P, Ganesh Kumari K, Lakshmi Prabha A, Pill-Soon Song and Jayabalan N (2009) Transgenic Indian Cotton (Gossypium hirsutum) Harboring Rice Chitinase Gene (Chi II) Confers Resistance to Two Fungal Pathogens. American J. Biochemistry and Biotechnology, 5 (2): 63-74. IF:1.03
11. Kumari KG, Ganesan M and N. Jayabalan (2008) Somatic organogenesis and plant regeneration in Ricinus communis. Biologia Plantarum, 52 (1) : 17-25. IF:1.55
12. Ganesan M, Chandrasekar R, Ranjitha Kumari BD and Jayabalan N (2007) Somatic embryogenesis and plant regeneration of okra (Abelmoschus esculentus (L.) Moench) through suspension culture. Biologia Plantarum, 51 (3) : 414 - 420. IF:1.55
13. Jayabalan N, Ganesan M (2007) Transformation of Indian cotton (Gossypium hirsutum) by using rice chitinase gene (Chi II) for enhanced resistance to fungal pathogens. Proc. Of the International Conference on Integration of Science & Technology for Sustainable Development, Bangkok, Thailand. 1 : 163 -172.
14. Ganesan M and Jayabalan N (2006) Isolation of disease tolerant cotton (Gossypium hirsutum L. cv. SVPR 2) plants by screening somatic embryos with fungal culture filtrate. Plant Cell Tissue and Organ Culture, 87 : 273 - 284. IF:2.0
15. Ganesan, M. and N. Jayabalan (2006). Influence of cytokinins, auxins and polyamines on in vitro mass multiplication of cotton (Gossypium hirsutum L. cv. SVPR2). Indian J. Experimental Biology, 44 : 506-513. IF:2.17
16. Ganesan M and Jayabalan N (2005). In vitro plant regeneration from the callus of shoot tips in cotton (Gossypium hirsutum L. cv. SVPR 2). Iranian J. Biotechnology, 3 (3) : 144 - 151.
17. Ganesan M and Jayabalan N (2005). Carbon source dependent somatic embryogenesis and plant regeneration in cotton cv SVPR2. Indian J. Experimental Biology, 43 : 921 – 925. IF:2.17
18. Bhanumathi P, Ganesan M and Jayabalan N (2005). A simple and improved protocol for direct and indirect somatic embryogenesis of peanut (Arachis hypogaea L.). J. Agricultural Technology, 1: 327 – 344.
19. Ganesan M, Bhanumathi P and Jayabalan N (2005). Mutagenic effect of sodium azide on somatic embryo regeneration and root growth of cotton (Gossypium hirsutum L. cv. SVPR2). J. Agricultural Technology, 1 : 365 – 380.
20. Ganesan M and Jayabalan N (2004). Haemoglobin enhances somatic embryogenesis and plant regeneration. Agricell Report, 43 (5) : 36.
21. Ganesan M and Jayabalan N (2004). Evaluation of Haemoglobin for improved somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum cv SVPR 2). Plant Cell Reports, 23 : 181 – 187. IF :2.86